Mentors at Your Benchside

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Bitesize Bio hosts webinars on a range of topics, from microscopy to genomics, in order to help you succeed at the bench. The Mentors at the Benchside podcast brings the “missing lab manual for bioscientists” to your ears. The original webinars can be found at http://bitesizebio.com/webinars/

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Website
http://www.bitesizebio.com
Description
Bioscience Research Tips and Advice Webinars from Bitesize Bio
Language
🇬🇧 English
last modified
2019-01-06 04:47
last episode published
2018-11-27 18:45
publication frequency
29.96 days
Contributors
Bitesize Bio author  
Amanda Welch owner  
Explicit
false
Number of Episodes
54
Rss-Feeds
Detail page
Categories
Science & Medicine Natural Sciences Medicine

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Episodes

Date Thumb Title & Description Contributors
27.11.2018

Array Tomography for SEM 3D Reconstruction

Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tr...
20.11.2018

Expanding the limits of EM sample prep with ICE

In this webinar you will learn: - Improved vitrification: Specimen vitrification without synchronisation fluid - Vitrification strategies: Optimized freezing for different techniques - Light and electrical stimulation: Dissect cellular processes with ...
13.11.2018

Using CRISPR/Cas9 to detect DNA sequences with AFM

Join Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging. In this webinar, you will learn: 1. How CRISPR/Cas9 can be use...
1.11.2018

How to Use CRISPR to Accelerate Cancer Therapies

Join Theo Roth as he describes his lab's novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific si...
16.10.2018

Nucleic acids 101: Confirming Their Quality

Join us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn: - How to choose the best method to extract your nucleic acids - Which method you should chose to determine nucleic ...
10.10.2018

Live Cell Isolation by Laser Microdissection

In this webinar, you will learn: - How to best prepare your specimen for live cell isolation by laser microdissection - How to optimize your laser microdissection workflow - How to avoid common pitfalls of this technique Laser microdissection is a to...
9.10.2018

Reliable quantification of fluorescence images

In this webinar, you will learn: - How to overcome issues with stage drift and crosstalk between channels - How to obtain reliable images and reproducible quantification results - How to proceed with advanced image analysis after restoring images Dur...
27.09.2018

Crash Course: Developing a Fool-Proof ELISA

Join us in this webinar featuring Dr. Omonse Talton who will guide you through developing a "fool proof" enzyme-linked immunosorbent assay (ELISA). In this webinar, you will learn: - When and why you should use the different types of ELISAs– direct, i...
20.09.2018

Discovering PARP inhibitor resistance mechanisms with CRISPR

In this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide muta...
18.09.2018

14 Ways to Measure Immune Cell Activation

Activation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease. Ther...
12.09.2018

A Guide to Target Gene Validation Using Quantitative RT-PCR

While next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In ...
5.09.2018

How to Ensure Your Cell-Based Assays Are Reproducible

Join us in this webinar featuring Dr. Vicki Doronina as she takes you through vital components of assay design. In this webinar you will learn: How to choose between immortalized cells and primary cells for your assay How to avoid sources of bias in y...
22.08.2018

Simultaneous Proteomics and Transcriptomics

Join us in this webinar featuring Dr. Marlon Stoeckius as he explains how you can improve your single-cell RNA-sequencing (scRNA-seq) experiments. In this webinar, you will learn: - How you can run one scRNA-seq experiment with numerous protein marker...
11.07.2018

Using qPCR to Validate Epigenetic Enrichment of Pathogen DNA

qPCR is one of the most specific and sensitive tools in molecular biology, allowing the quantification of target DNA molecules present at less than 1 in 106. Next Generation Sequencing (NGS) has similar potential. However, the presence of large amounts...
10.07.2018

Super-resolved STED spectroscopy: New insights

Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifi...
28.06.2018

Challenges and solutions in microbiome analysis workflows

Elucidating meaningful, unbiased microbial community profiles from complex microbiome samples is challenging. In this webinar, you will learn: – the sources of bias throughout the microbiome analysis workflow – practical solutions for troubleshooting ...
26.06.2018

Precise and fast spectral FLIM at video rate in confocal

Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the ratio of the donor and acceptor intensit...
29.05.2018

Complete and Fast 3D Image Analysis in Microscopy

If image analysis is a place you fear to tread, or if you struggle with over complicated and time-consuming microscopy image analysis workflows, this is your opportunity to go beyond those limits. You will learn a fast, efficient and flexible approach...
15.05.2018

A Guide to Harnessing DNA Assembly for Drug Discovery

Current DNA synthesis and assembly technologies give today's genetic engineers unprecedented freedom to control every aspect of genetic design. In this webinar on DNA assembly, you will learn: - Key concepts in DNA assembly - The importance of analyzi...
24.04.2018

Get the Best Microscopy Images with Huygens Batch Express

In this webinar, you will learn about the advantages of deconvolving all your images fully automatically. Discussed in this webinar: - How to improve the quality of your images with the new Batch Express - How to increase resolution and signal of all ...
17.04.2018

LASSO Probes for Massively Multiplexed Cloning

In this webinar, you will learn how to solve a major problem in creating expression libraries from genome sequences for downstream analyses. Specifically, you will learn: - The difference and benefits of LASSO cloning over NGS - How LASSO cloning allo...
11.12.2017

2D Superresolution for ZEISS Airyscan with 120 nm resolution

Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel function...
15.11.2017

Designing Flanking Homology DNA Assembly Experiments

Join us in this webinar on demystifying DNA assembly. In this webinar, you will learn: - How flanking homology DNA assembly methods work - How to use web-based software to design experimental methods for flanking homology DNA assembly methods - How sy...
8.11.2017

Biological Applications of XRM and Correlative XRM – FIB-SEM

In this webinar, you will learn about the power of 3D imaging of larger samples. In particular, you will: - Gain insight in to how to use x-ray microscopy to analyze biological samples - Learn the basics about sample preparation for x-ray microscopy -...
8.09.2017

Large Volume Serial Blockface Electron Microscopy Imaging

An unlikely renaissance is occurring in biological imaging, propelled by the ongoing development of serial blockface electron microscopy imaging technologies. Imaging systems based on scanning EM instruments place large-volume automated EM serial imagi...
20.07.2017

Optocardiography: Optical Imaging of Cardiac Physiology

In this webinar, you will learn how to use optical imaging for functional cardiac physiological mapping of transmembrane potential, calcium handling, and metabolism. The main aspects covered in the webinar include: - How to design an effective optoca...
12.06.2017

How to Conduct Localized Proteomics of Microscopic Regions

In this webinar, you will learn: - How to perform isolated proteomics of microscopic regions of interest - How to use this technique with FFPE tissue - How proteomics can help you understand molecular mechanisms in disease We recently developed a tec...
7.06.2017

Harnessing the Power of Heterogeneity in cryo-EM

A major impediment in the determination of high resolution protein structures by single particle cryo-electron microscopy has been the presence of sample heterogeneity. Oftentimes, heterogeneity is due the dynamic nature of protein complexes, which can...
18.05.2017

qPCR and RNAseq: The Battle of the Strands!

In this webinar, you will learn how qPCR can be used in combination with new technologies, like RNAseq, and how this will help you in your research. The main aspects to be covered in this webinar include: - When you should choose RNA-seq over qPCR al...
16.05.2017

Mouse Whole-Brain Volume Electron Microscopy for Cellular Co

Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These...
10.05.2017

Engineering Cell Behavior with Synthetic Biology

In this webinar, you will learn how synthetic biology can be used to engineer desirable and robust behaviors or traits into model organisms. The main aspects to be covered in this webinar include: - Introduction to synthetic biology – what it is and ...
3.05.2017

Development of a Potential Recombinant Protein Vaccine in E.

Recombinant proteins can be powerful stimulators of the immune system making them good candidates for vaccines. Using the intracellular parasite, Leishmania donovani as an example, this webinar will take you through the steps required for development a...
18.04.2017

Guide to CRISPR/Cas9 Delivery: How to Maximize Your Editing

In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research. The main points in the webinar will include: - How to design guide RNAs using online tools specific to ...
1.12.2016

Multiplex PCR Technology: What Is It All About?

Join Dr. Karen O’Hanlon Cohrt for a practical tour of multiplex PCR technology, where you will learn the following and much more: Principle behind multiplex PCR technology Popular applications of this technology How to set up this reaction Advantage...
25.10.2016

Open Access: Facts, Myths, and Effects on Your Research Fund

The rise of open access is changing how research is communicated. In this webinar, we’ll celebrate Open Access Week 2016 by taking a closer look at how open access affects how researchers write and publish their results. Specifically, we will: Define ...
29.09.2016

Color Image Quality Control in Microscopy

In this webinar, Mark Clymer discusses color reproduction in microscopy images—and how to fix it if your color reproduction goes awry.
28.09.2016

Incorporating New Dyes to Simplify Panel Design - Reagent

Incorporating New Dyes to Simplify Panel Design: Accelerating Reagent Availability In this webinar you will learn how custom reagents can aid you in flow cytometry panel design—without long waits for generating these components. We will cover: How q...
9.08.2016

Decoding Cancer: Practical Advice for Working with Cellular

In this webinar, Dr. Ania Wronski discusses the process for genotyping cells—particularly tumor cells—and practical advice for analyzing the resulting data.
22.07.2016

All about miRNAs: Practical Tips, Advice, and Applications

Dr. Brian Adams is a researcher on the leading edge of miRNA research. Join him in this webinar as he demystifies working with miRNAs and gives you his best tips and tricks for working with them in your research.
21.06.2016

Tools for Analyzing Genetic Variants from Sequencing Data

Every individual harbors millions of genetic variants, many of which may contribute to phenotype. As the cost of sequencing has plummeted, we now have the opportunity to profile this variation across thousands of individuals. Here I will provide an ove...
17.06.2016

5 Actionable Steps for Transitioning to a Non-faculty Career

The current state of the academic job market has come under scrutiny in recent years, largely because of the shrinking number of tenure-track jobs available. This situation has forced graduate students and postdocs to reconsider their employment option...
17.06.2016

Next-Generation Sequencing Overview: Step By Step Guide

Next generation sequencing (NGS) is a powerful tool for investigating genome-wide phenomena. It has been used to study epigenetic profiles as part of the ENCODE project. It is being used to understand the genetic basis of both common and rare diseases....
17.06.2016

How Bitesize Bio Gets Progressive Companies to Help You

Whether it’s equipment costing hundreds of thousands of dollars and requiring millions of dollars in R&D investments or standardized kits that relieve you of tedious grunt work day in and day out, you rely on the support of companies like our spons...
17.06.2016

Using FIT probes and Super-Resolution Microscopy

For more information, please visit: http://bitesizebio.com/webinar/25961/deciphering-steps-of-mrnp-assembly-in-developing-oocytes-using-super-resolution-microscopy/ All mRNA molecules recruit specific proteins to form ribonucleoprotein complexes (mRNP...
17.06.2016

Know the Players: Combinatorial, Single-Cell Approaches

We know that cellular heterogeneity in the tumor microenvironment complicates the diagnosis and treatment of cancer. Tumors are complex, dynamic systems composed of diverse cell types in various functional states, including cancer cells and infiltratin...
17.06.2016

Critical Success Factors for Sample Collection

Biobanks, or biorepositories, store biological samples that are critical for research in the biomedical fields. Samples from biobanks have been used for genomics research, investigations into the development of personalized medicine, and treatment of d...
17.06.2016

Laser Microdissection – Dissection Perfection

Leica Microsystems Laser Microdissection systems are the method of choice for laser-based microscopic sample dissection and collection. Laser Microdissection (LMD) is a microscopic technique for isolating homogeneous, specific and pure targets from het...
17.06.2016

How To Be Happier and More Productive in Your Work

Enjoying your work should be one of life’s top priorities not only because you spend much of your time working, but also because if you enjoy your work you’ll inevitably do a better job. Having worked in an uptight academic lab, a very laid-back Danis...
15.06.2016

Getting Correct Color from Your Camera

Seeing and believing that the dress is yellow may not be that important for the casual wedding image, but in microscopy imaging seeing and believing is critical. Microscopy images that contain color, such as images from histology­-stained specimens, mu...
15.06.2016

Combining Light Sheet & Confocal Microscopy

Living cells and organisms often suffer from the high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signa...